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In order to define triglyceride compositions in fat and oil triglycerides were separated by thin layer chromatography (TLC) from corn oil, and the separated triglycerides were graduated according to each partition number(PN) by high performance liquid chromatography (HPLC) using column of μ-Bondapack C_(18) and each graduation was graduated again according to acyl carbon number by gas liquid chromatography(GLC). Fatty acid compositions were analyzed by GLC after their graduation were methylated in according to PN. The triglyceride compositions were estimated by synthesizing the above three results. The estimated triglycerides consisted of 36 kinds in corn oil. The major triglyceride compositions of sample oil were as follows: 21.5%. (C(18:2), C(18:2), C(18:1)), 17.4%(C(18:1), C(18:2), C(18:1)) 15.4% (C(18:1), C(18:2), C(16:0)), 11.1% (C(16:0), C(18:2), C(18:2)), 9.0% (C(18:1), C(18:1), C(18:1)). 8.0% (C(18:2), C(18:2), C(18:2)), 5.7% (C(18:1), C(18:1), C(16:0)), 2.2% (C(16:0), C(16:0), C(18:2)), 1.6% (C(18:2), C(18:0), C(18:1)), 1.1% (C(18:2), C(18:0), C(16:0)), 1.1% (C(16:0), C(16:0), C(18:1)).
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